This function obtains the count data from the raw Median Fluorescent Intensity (MFI). This function relies on the output of the Antigen-specific counts (`getAntigenCounts`) and the Well or Sample-specific counts (`getCounts`).
Examples
# \donttest{
# Step 0: Load example raw data
your_raw_data <- c(
system.file("extdata", "example_MAGPIX_plate1.csv", package = "SeroTrackR"),
system.file("extdata", "example_MAGPIX_plate2.csv", package = "SeroTrackR")
)
your_plate_layout <- system.file(
"extdata",
"example_platelayout_1.xlsx",
package = "SeroTrackR"
)
# Step 1: Read serology data and plate layout
sero_data <- readSeroData(your_raw_data,"magpix")
#> PASS: File example_magpix_plate1.csv successfully validated.
#> PASS: File example_magpix_plate2.csv successfully validated.
plate_list <- readPlateLayout(your_plate_layout, sero_data)
#> Plate layouts correctly identified!
# Step 2: Process counts and perform quality control
counts <- processCounts(sero_data)
counts_raw <- getCounts(counts)
sample_ids <- getSampleID(counts, plate_list)
antigen_cts <- getAntigenCounts(counts, plate_list)
counts_qc <- getCountsQC(antigen_cts, counts_raw)
# }
