A master function combining the entire PvSeroApp pipeline into one command to run in R.
Usage
runPvSeroPipeline(
raw_data,
plate_layout,
platform = "magpix",
location,
experiment_name = "experiment1",
std_point = 10,
classify = "Yes",
algorithm_type = "antibody_model",
sens_spec = "balanced"
)Arguments
- raw_data
String with the raw data path.
- plate_layout
An ".xlsx" file with sheets labelled plate1, plate2... etc.
- platform
"magpix" or "bioplex". Default = "magpix".
- location
"PNG" or "ETH" to filter WEHI standard curve data.
- experiment_name
User-input experiment name.
- std_point
Standard Point Curve: 5 = 5-point curve, 10 = 10-point curve, "PvLDH" for LDH specific curve. Default = 10. Value is an integer.
- classify
"Yes" or "No" depending on whether you would like classification or not. Default = "Yes".
- algorithm_type
User-selected algorithm choice: - "antibody_model" (PvSeroTaT model; default), or - "antibody_model_excLF016" (PvSeroTat excluding LF016).
- sens_spec
User-selected Sensitivity/Specificity threshold: - "balanced" (default), - "85% sensitivity", - "90% sensitivity", - "95% sensitivity", - "85% specificity", - "90% specificity". - "95% specificity".
Value
classifyResults_output, stdcurve_plot, plateqc_plot, check_repeats_output, blanks_plot, model_plot
Examples
# \donttest{
# Example data supplied with the package
your_raw_data <- c(
system.file("extdata", "example_MAGPIX_plate1.csv", package = "SeroTrackR"),
system.file("extdata", "example_MAGPIX_plate2.csv", package = "SeroTrackR"),
system.file("extdata", "example_MAGPIX_plate3.csv", package = "SeroTrackR")
)
plate_layout <- system.file(
"extdata", "example_platelayout_1.xlsx", package = "SeroTrackR"
)
# Run full pipeline including classification
runPvSeroPipeline(
raw_data = your_raw_data,
plate_layout = plate_layout,
platform = "magpix",
location = "PNG",
experiment_name = "experiment1",
std_point = 10,
algorithm_type = "antibody_model",
sens_spec = "balanced",
classify = "Yes"
)
#> PASS: File example_magpix_plate1.csv successfully validated.
#> PASS: File example_magpix_plate2.csv successfully validated.
#> PASS: File example_magpix_plate3.csv successfully validated.
#> Plate layouts correctly identified!
#> QC Processes completed.
#> MFI to RAU conversion completed.
#> Please write a standard point curve.
#> QC Plotting completed.
#> Pv classification completed.
#> [[1]]
#> # A tibble: 252 × 12
#> SampleID Plate QC_total PvEBP `Pv-fam-a` PvMSP5 `PvMSP1-19` PvMSP8
#> <chr> <chr> <chr> <dbl> <dbl> <dbl> <dbl> <dbl>
#> 1 ABC013 plate1 pass 0.000818 0.00140 0.000218 0.000131 0.0000544
#> 2 ABC097 plate2 pass 0.000935 0.00140 0.000213 0.000122 0.0000447
#> 3 ABC181 plate3 pass 0.000925 0.00148 0.000210 0.000125 0.0000532
#> 4 ABC022 plate1 pass 0.02 0.0194 0.000814 0.00342 0.000700
#> 5 ABC106 plate2 pass 0.02 0.0166 0.000821 0.00337 0.000672
#> 6 ABC190 plate3 pass 0.02 0.0189 0.000817 0.00383 0.000661
#> 7 ABC023 plate1 pass 0.000247 0.00668 0.000136 0.000111 0.000154
#> 8 ABC107 plate2 pass 0.000268 0.00638 0.000125 0.000101 0.000143
#> 9 ABC191 plate3 pass 0.000267 0.00716 0.000131 0.000107 0.000144
#> 10 ABC024 plate1 pass 0.000466 0.000351 0.000286 0.000117 0.0000897
#> # ℹ 242 more rows
#> # ℹ 4 more variables: PvRBP2b <dbl>, PvPTEX150 <dbl>, PvCSS <dbl>,
#> # pred_class_max <fct>
#>
#> [[2]]
#> NULL
#>
#> [[3]]
#>
#> [[4]]
#> # A tibble: 2 × 4
#> Location SampleID Plate QC
#> <chr> <chr> <chr> <chr>
#> 1 A1 Blank1 plate2 fail
#> 2 A2 Blank2 plate2 fail
#>
#> [[5]]
#>
#> [[6]]
#> [[6]]$`1`
#>
#> [[6]]$`2`
#>
#> [[6]]$`3`
#>
#>
# Run processing pipeline only (no classification)
runPvSeroPipeline(
raw_data = your_raw_data,
plate_layout = plate_layout,
platform = "magpix",
location = "PNG",
experiment_name = "experiment1",
std_point = 10,
algorithm_type = "antibody_model",
sens_spec = "balanced",
classify = "No"
)
#> PASS: File example_magpix_plate1.csv successfully validated.
#> PASS: File example_magpix_plate2.csv successfully validated.
#> PASS: File example_magpix_plate3.csv successfully validated.
#> Plate layouts correctly identified!
#> QC Processes completed.
#> MFI to RAU conversion completed.
#> Please write a standard point curve.
#> QC Plotting completed.
#> No Classification Performed.
#> [[1]]
#> SampleID Plate PvEBP_MFI PvEBP_Dilution Pv-fam-a_MFI Pv-fam-a_Dilution
#> 1 ABC013 plate1 2712.0 8.179565e-04 1569.0 1.399418e-03
#> 2 ABC014 plate1 134.0 5.055201e-05 378.0 3.776621e-04
#> 3 ABC015 plate1 182.0 6.726976e-05 209.0 2.218882e-04
#> 4 ABC016 plate1 152.0 5.695020e-05 229.5 2.415574e-04
#> 5 ABC017 plate1 1135.0 3.415765e-04 236.0 2.477351e-04
#> 6 ABC018 plate1 174.0 6.455514e-05 395.0 3.927494e-04
#> 7 ABC019 plate1 421.0 1.413090e-04 2081.5 1.860753e-03
#> 8 ABC020 plate1 24.0 1.953125e-05 49.0 5.079836e-05
#> 9 ABC021 plate1 24.0 1.953125e-05 45.0 4.575984e-05
#> 10 ABC022 plate1 21464.0 2.000000e-02 11789.0 1.939927e-02
#> 11 ABC023 plate1 795.0 2.470670e-04 6135.5 6.684025e-03
#> 12 ABC024 plate1 1574.0 4.661020e-04 348.5 3.512927e-04
#> 13 ABC025 plate1 146.0 5.483661e-05 408.5 4.046797e-04
#> 14 ABC026 plate1 1330.5 3.965094e-04 3036.0 2.790879e-03
#> 15 ABC027 plate1 358.0 1.226868e-04 4074.5 3.930596e-03
#> 16 ABC028 plate1 481.0 1.587103e-04 1870.0 1.667559e-03
#> 17 ABC029 plate1 551.0 1.787133e-04 1282.0 1.150111e-03
#> 18 ABC030 plate1 943.0 2.881465e-04 584.5 5.572765e-04
#> 19 ABC031 plate1 532.5 1.734526e-04 1533.0 1.367833e-03
#> 20 ABC032 plate1 768.0 2.395611e-04 12605.0 2.000000e-02
#> 21 ABC033 plate1 130.0 4.910554e-05 239.5 2.510506e-04
#> 22 ABC034 plate1 40.0 1.953125e-05 60.0 6.432704e-05
#> 23 ABC035 plate1 167.0 6.215858e-05 605.0 5.748048e-04
#> 24 ABC036 plate1 1879.0 5.556667e-04 4562.0 4.518459e-03
#> 25 ABC037 plate1 256.0 9.139463e-05 1507.0 1.345082e-03
#> 26 ABC038 plate1 24.0 1.953125e-05 49.0 5.079836e-05
#> 27 ABC039 plate1 384.0 1.304232e-04 1708.0 1.522310e-03
#> 28 ABC040 plate1 210.0 7.658872e-05 512.0 4.949525e-04
#> 29 ABC041 plate1 351.5 1.207401e-04 312.0 3.182808e-04
#> 30 ABC042 plate1 1278.0 3.816873e-04 5810.0 6.199666e-03
#> 31 ABC043 plate1 318.0 1.106166e-04 397.0 3.945196e-04
#> 32 ABC044 plate1 493.5 1.623030e-04 2980.5 2.733931e-03
#> 33 ABC045 plate1 9686.0 5.163493e-03 873.0 8.021047e-04
#> 34 ABC046 plate1 777.5 2.422029e-04 581.0 5.542802e-04
#> 35 ABC047 plate1 455.0 1.512035e-04 416.5 4.117296e-04
#> 36 ABC048 plate1 202.0 7.395303e-05 1142.0 1.030285e-03
#> 37 ABC049 plate1 333.5 1.153202e-04 623.5 5.905944e-04
#> 38 ABC050 plate1 561.0 1.815503e-04 7984.0 9.856092e-03
#> 39 ABC051 plate1 506.5 1.660292e-04 340.0 3.436458e-04
#> 40 ABC052 plate1 254.0 9.076186e-05 367.0 3.678588e-04
#> 41 ABC053 plate1 888.0 2.728843e-04 1955.0 1.744700e-03
#> 42 ABC054 plate1 426.0 1.427701e-04 1877.0 1.673887e-03
#> 43 ABC055 plate1 5733.0 2.097090e-03 1301.5 1.166883e-03
#> 44 ABC056 plate1 19092.0 2.000000e-02 14422.0 2.000000e-02
#> 45 ABC057 plate1 596.0 1.914462e-04 15139.0 2.000000e-02
#> 46 ABC058 plate1 1028.0 3.117588e-04 2646.0 2.398507e-03
#> 47 ABC059 plate1 512.0 1.676026e-04 4735.0 4.735904e-03
#> 48 ABC060 plate1 883.0 2.714969e-04 1953.0 1.742877e-03
#> 49 ABC061 plate1 977.0 2.975861e-04 8719.0 1.134508e-02
#> 50 ABC062 plate1 791.0 2.459555e-04 1944.0 1.734680e-03
#> 51 ABC063 plate1 397.0 1.342634e-04 987.0 8.985851e-04
#> 52 ABC064 plate1 1111.0 3.348763e-04 613.0 5.816359e-04
#> 53 ABC065 plate1 956.0 2.917552e-04 2369.0 2.130408e-03
#> 54 ABC066 plate1 1307.0 3.898677e-04 20590.0 2.000000e-02
#> 55 ABC067 plate1 433.0 1.448121e-04 382.0 3.812188e-04
#> 56 ABC068 plate1 161.0 6.008761e-05 279.0 2.879920e-04
#> 57 ABC069 plate1 439.0 1.465590e-04 732.5 6.832185e-04
#> 58 ABC070 plate1 1837.0 5.431545e-04 11406.0 1.820423e-02
#> 59 ABC071 plate1 244.0 8.758422e-05 1665.5 1.484571e-03
#> 60 ABC072 plate1 482.0 1.589981e-04 3990.5 3.832880e-03
#> 61 ABC073 plate1 656.0 2.083103e-04 3103.0 2.860133e-03
#> 62 ABC074 plate1 243.0 8.726515e-05 568.5 5.435700e-04
#> 63 ABC075 plate1 1409.5 4.189266e-04 1123.0 1.014094e-03
#> 64 ABC076 plate1 467.5 1.548185e-04 6741.0 7.640785e-03
#> 65 ABC077 plate1 329.0 1.139583e-04 630.0 5.961363e-04
#> 66 ABC078 plate1 1122.5 3.380858e-04 17810.0 2.000000e-02
#> 67 ABC079 plate1 10853.0 6.601406e-03 817.5 7.551680e-04
#> 68 ABC080 plate1 743.5 2.327430e-04 820.0 7.572825e-04
#> 69 ABC081 plate1 723.0 2.270316e-04 1255.0 1.126924e-03
#> 70 ABC082 plate1 168.0 6.250221e-05 684.0 6.420759e-04
#> 71 ABC083 plate1 180.0 6.659345e-05 276.0 2.852145e-04
#> 72 ABC084 plate1 249.5 8.933482e-05 4104.0 3.965157e-03
#> 73 ABC085 plate1 479.0 1.581345e-04 190.0 2.033772e-04
#> 74 ABC086 plate1 164.0 6.112508e-05 327.0 3.319039e-04
#> 75 ABC087 plate1 281.5 9.938764e-05 1794.0 1.599135e-03
#> 76 ABC088 plate1 269.0 9.548627e-05 1067.0 9.664571e-04
#> 77 ABC089 plate1 3247.0 1.003038e-03 382.0 3.812188e-04
#> 78 ABC090 plate1 20593.0 2.000000e-02 14902.5 2.000000e-02
#> 79 ABC091 plate1 395.0 1.336737e-04 9914.0 1.409531e-02
#> 80 ABC092 plate1 904.5 2.774625e-04 1871.0 1.668463e-03
#> 81 ABC093 plate1 323.0 1.121378e-04 2007.5 1.792680e-03
#> 82 ABC094 plate1 706.0 2.222901e-04 2087.0 1.865834e-03
#> 83 ABC095 plate1 323.0 1.121378e-04 4950.0 5.012861e-03
#> 84 ABC096 plate1 367.0 1.253737e-04 1067.0 9.664571e-04
#> 85 ABC097 plate2 2712.0 9.346332e-04 1569.0 1.402076e-03
#> 86 ABC098 plate2 134.0 4.777942e-05 378.0 3.692781e-04
#> 87 ABC099 plate2 182.0 6.539640e-05 209.0 2.129087e-04
#> 88 ABC100 plate2 152.0 5.446912e-05 229.5 2.325230e-04
#> 89 ABC101 plate2 1135.0 3.779987e-04 236.0 2.386938e-04
#> 90 ABC102 plate2 174.0 6.250694e-05 395.0 3.845120e-04
#> 91 ABC103 plate2 421.0 1.469246e-04 2081.5 1.862236e-03
#> 92 ABC104 plate2 24.0 1.953125e-05 49.0 4.634429e-05
#> 93 ABC105 plate2 24.0 1.953125e-05 45.0 4.166565e-05
#> 94 ABC106 plate2 21464.0 2.000000e-02 11789.0 1.661216e-02
#> 95 ABC107 plate2 795.0 2.679831e-04 6135.5 6.384115e-03
#> 96 ABC108 plate2 1574.0 5.238400e-04 348.5 3.426795e-04
#> 97 ABC109 plate2 146.0 5.225139e-05 408.5 3.965653e-04
#> 98 ABC110 plate2 1330.5 4.422770e-04 3036.0 2.774190e-03
#> 99 ABC111 plate2 358.0 1.260446e-04 4074.5 3.863675e-03
#> 100 ABC112 plate2 481.0 1.665902e-04 1870.0 1.670155e-03
#> 101 ABC113 plate2 551.0 1.893471e-04 1282.0 1.151365e-03
#> 102 ABC114 plate2 943.0 3.156767e-04 584.5 5.510978e-04
#> 103 ABC115 plate2 532.5 1.833481e-04 1533.0 1.370389e-03
#> 104 ABC116 plate2 768.0 2.592973e-04 12605.0 1.861550e-02
#> 105 ABC117 plate2 130.0 4.627728e-05 239.5 2.420075e-04
#> 106 ABC118 plate2 40.0 1.953125e-05 60.0 5.902633e-05
#> 107 ABC119 plate2 167.0 5.996461e-05 605.0 5.688743e-04
#> 108 ABC120 plate2 1879.0 6.288078e-04 4562.0 4.414393e-03
#> 109 ABC121 plate2 256.0 9.146063e-05 1507.0 1.347550e-03
#> 110 ABC122 plate2 24.0 1.953125e-05 49.0 4.634429e-05
#> 111 ABC123 plate2 384.0 1.346960e-04 1708.0 1.525150e-03
#> 112 ABC124 plate2 210.0 7.538779e-05 512.0 4.879220e-04
#> 113 ABC125 plate2 351.5 1.238732e-04 312.0 3.094382e-04
#> 114 ABC126 plate2 1278.0 4.249198e-04 5810.0 5.951118e-03
#> 115 ABC127 plate2 318.0 1.126204e-04 397.0 3.863001e-04
#> 116 ABC128 plate2 493.5 1.706665e-04 2980.5 2.718960e-03
#> 117 ABC129 plate2 9686.0 5.345379e-03 873.0 7.994295e-04
#> 118 ABC130 plate2 777.5 2.623532e-04 581.0 5.480594e-04
#> 119 ABC131 plate2 455.0 1.580901e-04 416.5 4.036906e-04
#> 120 ABC132 plate2 202.0 7.255118e-05 1142.0 1.030412e-03
#> 121 ABC133 plate2 333.5 1.178403e-04 623.5 5.848896e-04
#> 122 ABC134 plate2 561.0 1.925860e-04 7984.0 9.125684e-03
#> 123 ABC135 plate2 506.5 1.748995e-04 340.0 3.349733e-04
#> 124 ABC136 plate2 254.0 9.076932e-05 367.0 3.593854e-04
#> 125 ABC137 plate2 888.0 2.979293e-04 1955.0 1.746960e-03
#> 126 ABC138 plate2 426.0 1.485705e-04 1877.0 1.676461e-03
#> 127 ABC139 plate2 5733.0 2.359771e-03 1301.5 1.168273e-03
#> 128 ABC140 plate2 19092.0 2.000000e-02 14422.0 2.000000e-02
#> 129 ABC141 plate2 596.0 2.039039e-04 15139.0 2.000000e-02
#> 130 ABC142 plate2 1028.0 3.431865e-04 2646.0 2.392064e-03
#> 131 ABC143 plate2 512.0 1.766886e-04 4735.0 4.616269e-03
#> 132 ABC144 plate2 883.0 2.963175e-04 1953.0 1.745147e-03
#> 133 ABC145 plate2 977.0 3.266673e-04 8719.0 1.036243e-02
#> 134 ABC146 plate2 791.0 2.666962e-04 1944.0 1.736992e-03
#> 135 ABC147 plate2 397.0 1.390028e-04 987.0 8.971839e-04
#> 136 ABC148 plate2 1111.0 3.701703e-04 613.0 5.758028e-04
#> 137 ABC149 plate2 956.0 3.198771e-04 2369.0 2.128806e-03
#> 138 ABC150 plate2 1307.0 4.344983e-04 20590.0 2.000000e-02
#> 139 ABC151 plate2 433.0 1.508725e-04 382.0 3.728684e-04
#> 140 ABC152 plate2 161.0 5.777447e-05 279.0 2.790082e-04
#> 141 ABC153 plate2 439.0 1.528434e-04 732.5 6.788558e-04
#> 142 ABC154 plate2 1837.0 6.141516e-04 11406.0 1.572584e-02
#> 143 ABC155 plate2 244.0 8.730328e-05 1665.5 1.487393e-03
#> 144 ABC156 plate2 482.0 1.669165e-04 3990.5 3.771414e-03
#> 145 ABC157 plate2 656.0 2.232560e-04 3103.0 2.841251e-03
#> 146 ABC158 plate2 243.0 8.695578e-05 568.5 5.371994e-04
#> 147 ABC159 plate2 1409.5 4.685412e-04 1123.0 1.014048e-03
#> 148 ABC160 plate2 467.5 1.621805e-04 6741.0 7.227633e-03
#> 149 ABC161 plate2 329.0 1.163274e-04 630.0 5.905111e-04
#> 150 ABC162 plate2 1122.5 3.739198e-04 17810.0 2.000000e-02
#> 151 ABC163 plate2 10853.0 6.583714e-03 817.5 7.518379e-04
#> 152 ABC164 plate2 743.5 2.514166e-04 820.0 7.539823e-04
#> 153 ABC165 plate2 723.0 2.448221e-04 1255.0 1.127981e-03
#> 154 ABC166 plate2 168.0 6.032863e-05 684.0 6.371156e-04
#> 155 ABC167 plate2 180.0 6.467559e-05 276.0 2.762216e-04
#> 156 ABC168 plate2 249.5 8.921159e-05 4104.0 3.896256e-03
#> 157 ABC169 plate2 479.0 1.659374e-04 190.0 1.945000e-04
#> 158 ABC170 plate2 164.0 5.887084e-05 327.0 3.231474e-04
#> 159 ABC171 plate2 281.5 1.002235e-04 1794.0 1.601909e-03
#> 160 ABC172 plate2 269.0 9.593949e-05 1067.0 9.658776e-04
#> 161 ABC173 plate2 3247.0 1.148112e-03 382.0 3.728684e-04
#> 162 ABC174 plate2 20593.0 2.000000e-02 14902.5 2.000000e-02
#> 163 ABC175 plate2 395.0 1.383410e-04 9914.0 1.257410e-02
#> 164 ABC176 plate2 904.5 3.032498e-04 1871.0 1.671056e-03
#> 165 ABC177 plate2 323.0 1.143069e-04 2007.5 1.794658e-03
#> 166 ABC178 plate2 706.0 2.393526e-04 2087.0 1.867275e-03
#> 167 ABC179 plate2 323.0 1.143069e-04 4950.0 4.872008e-03
#> 168 ABC180 plate2 367.0 1.290453e-04 1067.0 9.658776e-04
#> 169 ABC181 plate3 2712.0 9.246654e-04 1569.0 1.482532e-03
#> 170 ABC182 plate3 134.0 5.272190e-05 378.0 3.717704e-04
#> 171 ABC183 plate3 182.0 6.995153e-05 209.0 2.131843e-04
#> 172 ABC184 plate3 152.0 5.927566e-05 229.5 2.327960e-04
#> 173 ABC185 plate3 1135.0 3.740838e-04 236.0 2.389849e-04
#> 174 ABC186 plate3 174.0 6.713144e-05 395.0 3.874570e-04
#> 175 ABC187 plate3 421.0 1.491797e-04 2081.5 1.993909e-03
#> 176 ABC188 plate3 24.0 1.953125e-05 49.0 5.127838e-05
#> 177 ABC189 plate3 24.0 1.953125e-05 45.0 4.686218e-05
#> 178 ABC190 plate3 21464.0 2.000000e-02 11789.0 1.887078e-02
#> 179 ABC191 plate3 795.0 2.667857e-04 6135.5 7.155986e-03
#> 180 ABC192 plate3 1574.0 5.170860e-04 348.5 3.444706e-04
#> 181 ABC193 plate3 146.0 5.710479e-05 408.5 3.998936e-04
#> 182 ABC194 plate3 1330.5 4.370007e-04 3036.0 3.021103e-03
#> 183 ABC195 plate3 358.0 1.289165e-04 4074.5 4.261927e-03
#> 184 ABC196 plate3 481.0 1.682641e-04 1870.0 1.779709e-03
#> 185 ABC197 plate3 551.0 1.903542e-04 1282.0 1.206933e-03
#> 186 ABC198 plate3 943.0 3.132435e-04 584.5 5.610635e-04
#> 187 ABC199 plate3 532.5 1.845301e-04 1533.0 1.447563e-03
#> 188 ABC200 plate3 768.0 2.583339e-04 12605.0 2.000000e-02
#> 189 ABC201 plate3 130.0 5.124787e-05 239.5 2.423120e-04
#> 190 ABC202 plate3 40.0 1.953125e-05 60.0 6.327273e-05
#> 191 ABC203 plate3 167.0 6.464855e-05 605.0 5.797837e-04
#> 192 ABC204 plate3 1879.0 6.205657e-04 4562.0 4.892409e-03
#> 193 ABC205 plate3 256.0 9.532897e-05 1507.0 1.422381e-03
#> 194 ABC206 plate3 24.0 1.953125e-05 49.0 5.127838e-05
#> 195 ABC207 plate3 384.0 1.373128e-04 1708.0 1.618682e-03
#> 196 ABC208 plate3 210.0 7.969069e-05 512.0 4.948162e-04
#> 197 ABC209 plate3 351.5 1.268089e-04 312.0 3.105228e-04
#> 198 ABC210 plate3 1278.0 4.199942e-04 5810.0 6.657715e-03
#> 199 ABC211 plate3 318.0 1.158851e-04 397.0 3.893006e-04
#> 200 ABC212 plate3 493.5 1.722203e-04 2980.5 2.958522e-03
#> 201 ABC213 plate3 9686.0 5.659447e-03 873.0 8.252154e-04
#> 202 ABC214 plate3 777.5 2.613072e-04 581.0 5.578672e-04
#> 203 ABC215 plate3 455.0 1.600149e-04 416.5 4.072557e-04
#> 204 ABC216 plate3 202.0 7.692741e-05 1142.0 1.074966e-03
#> 205 ABC217 plate3 333.5 1.209529e-04 623.5 5.966773e-04
#> 206 ABC218 plate3 561.0 1.934990e-04 7984.0 1.031159e-02
#> 207 ABC219 plate3 506.5 1.763290e-04 340.0 3.365833e-04
#> 208 ABC220 plate3 254.0 9.465689e-05 367.0 3.616034e-04
#> 209 ABC221 plate3 888.0 2.959459e-04 1955.0 1.865244e-03
#> 210 ABC222 plate3 426.0 1.507769e-04 1877.0 1.786725e-03
#> 211 ABC223 plate3 5733.0 2.388051e-03 1301.5 1.225436e-03
#> 212 ABC224 plate3 19092.0 2.000000e-02 14422.0 2.000000e-02
#> 213 ABC225 plate3 596.0 2.044898e-04 15139.0 2.000000e-02
#> 214 ABC226 plate3 1028.0 3.400800e-04 2646.0 2.588978e-03
#> 215 ABC227 plate3 512.0 1.780655e-04 4735.0 5.123892e-03
#> 216 ABC228 plate3 883.0 2.943756e-04 1953.0 1.863223e-03
#> 217 ABC229 plate3 977.0 3.239615e-04 8719.0 1.173304e-02
#> 218 ABC230 plate3 791.0 2.655333e-04 1944.0 1.854133e-03
#> 219 ABC231 plate3 397.0 1.414923e-04 987.0 9.305112e-04
#> 220 ABC232 plate3 1111.0 3.664329e-04 613.0 5.870890e-04
#> 221 ABC233 plate3 956.0 3.173391e-04 2369.0 2.292616e-03
#> 222 ABC234 plate3 1307.0 4.293776e-04 20590.0 2.000000e-02
#> 223 ABC235 plate3 433.0 1.530107e-04 382.0 3.754642e-04
#> 224 ABC236 plate3 161.0 6.250829e-05 279.0 2.796256e-04
#> 225 ABC237 plate3 439.0 1.549234e-04 732.5 6.962936e-04
#> 226 ABC238 plate3 1837.0 6.060894e-04 11406.0 1.786398e-02
#> 227 ABC239 plate3 244.0 9.128662e-05 1665.5 1.576859e-03
#> 228 ABC240 plate3 482.0 1.685808e-04 3990.5 4.156476e-03
#> 229 ABC241 plate3 656.0 2.232901e-04 3103.0 3.097140e-03
#> 230 ABC242 plate3 243.0 9.094867e-05 568.5 5.464508e-04
#> 231 ABC243 plate3 1409.5 4.627584e-04 1123.0 1.057170e-03
#> 232 ABC244 plate3 467.5 1.639844e-04 6741.0 8.127056e-03
#> 233 ABC245 plate3 329.0 1.194841e-04 630.0 6.026131e-04
#> 234 ABC246 plate3 1122.5 3.700972e-04 17810.0 2.000000e-02
#> 235 ABC247 plate3 10853.0 7.087335e-03 817.5 7.741939e-04
#> 236 ABC248 plate3 743.5 2.506677e-04 820.0 7.764892e-04
#> 237 ABC249 plate3 723.0 2.442543e-04 1255.0 1.181364e-03
#> 238 ABC250 plate3 168.0 6.500416e-05 684.0 6.519428e-04
#> 239 ABC251 plate3 180.0 6.924820e-05 276.0 2.768054e-04
#> 240 ABC252 plate3 249.5 9.314233e-05 4104.0 4.299180e-03
#> 241 ABC253 plate3 479.0 1.676305e-04 190.0 1.948658e-04
#> 242 ABC254 plate3 164.0 6.357985e-05 327.0 3.244995e-04
#> 243 ABC255 plate3 281.5 1.038449e-04 1794.0 1.703844e-03
#> 244 ABC256 plate3 269.0 9.968233e-05 1067.0 1.004867e-03
#> 245 ABC257 plate3 3247.0 1.139162e-03 382.0 3.754642e-04
#> 246 ABC258 plate3 20593.0 2.000000e-02 14902.5 2.000000e-02
#> 247 ABC259 plate3 395.0 1.408501e-04 9914.0 1.426864e-02
#> 248 ABC260 plate3 904.5 3.011304e-04 1871.0 1.780711e-03
#> 249 ABC261 plate3 323.0 1.175225e-04 2007.5 1.918442e-03
#> 250 ABC262 plate3 706.0 2.389361e-04 2087.0 1.999541e-03
#> 251 ABC263 plate3 323.0 1.175225e-04 4950.0 5.417368e-03
#> 252 ABC264 plate3 367.0 1.318289e-04 1067.0 1.004867e-03
#> PvMSP5_MFI PvMSP5_Dilution PvMSP1-19_MFI PvMSP1-19_Dilution PvMSP8_MFI
#> 1 673.0 2.180089e-04 327.0 1.305912e-04 182.0
#> 2 117.0 4.190020e-05 197.0 8.294325e-05 58.0
#> 3 208.0 7.242988e-05 374.0 1.472327e-04 221.5
#> 4 101.0 3.632587e-05 89.0 4.000984e-05 48.0
#> 5 299.0 1.017565e-04 507.0 1.933202e-04 209.5
#> 6 175.0 6.154346e-05 78.0 3.531970e-05 70.0
#> 7 529.0 1.736206e-04 149.0 6.441513e-05 962.0
#> 8 22.0 1.953125e-05 13.0 1.953125e-05 15.5
#> 9 22.0 1.953125e-05 13.0 1.953125e-05 16.0
#> 10 2508.5 8.135025e-04 8001.0 3.415506e-03 2342.0
#> 11 407.0 1.357551e-04 273.0 1.111418e-04 535.5
#> 12 892.0 2.855092e-04 288.0 1.165852e-04 306.0
#> 13 1130.5 3.595999e-04 83.0 3.746198e-05 100.0
#> 14 965.0 3.080999e-04 174.0 7.415129e-05 79.0
#> 15 335.5 1.133199e-04 140.0 6.085706e-05 390.0
#> 16 421.0 1.401220e-04 182.5 7.741745e-05 1777.5
#> 17 331.5 1.120569e-04 165.0 7.066960e-05 77.0
#> 18 235.0 8.122279e-05 135.0 5.886686e-05 3017.0
#> 19 315.0 1.068364e-04 210.0 8.785222e-05 2575.5
#> 20 409.0 1.363794e-04 7632.0 3.202674e-03 9860.0
#> 21 72.0 2.598283e-05 64.0 2.921895e-05 37.0
#> 22 37.0 1.953125e-05 25.0 1.953125e-05 26.5
#> 23 253.0 8.703741e-05 329.0 1.313043e-04 3973.0
#> 24 415.0 1.382514e-04 18287.0 1.360075e-02 11482.5
#> 25 117.0 4.190020e-05 129.0 5.646513e-05 168.0
#> 26 22.0 1.953125e-05 12.0 1.953125e-05 15.0
#> 27 326.0 1.103187e-04 1172.5 4.162617e-04 986.0
#> 28 221.0 7.667494e-05 191.0 8.066345e-05 89.0
#> 29 165.5 5.837568e-05 88.0 3.958686e-05 66.0
#> 30 275.0 9.410003e-05 230.5 9.551526e-05 137.0
#> 31 130.0 4.637290e-05 130.5 5.706699e-05 78.0
#> 32 401.0 1.338813e-04 5932.5 2.310254e-03 3536.0
#> 33 333.0 1.125306e-04 166.0 7.105770e-05 791.0
#> 34 241.5 8.332655e-05 173.0 7.376566e-05 1479.5
#> 35 126.0 4.500165e-05 67.0 3.053985e-05 48.0
#> 36 137.5 4.893286e-05 176.0 7.492168e-05 204.5
#> 37 368.0 1.235483e-04 10430.0 5.013078e-03 2340.0
#> 38 441.0 1.463488e-04 128.0 5.606336e-05 141.0
#> 39 152.5 5.401250e-05 147.0 6.362705e-05 90.0
#> 40 392.5 1.312243e-04 89.0 4.000984e-05 142.5
#> 41 438.0 1.454156e-04 173.0 7.376566e-05 507.0
#> 42 186.0 6.519146e-05 9767.0 4.540744e-03 15945.0
#> 43 1920.5 6.135113e-04 220.5 9.178852e-05 4833.0
#> 44 1993.0 6.376207e-04 12836.5 6.996104e-03 7426.0
#> 45 371.5 1.246466e-04 229.0 9.495757e-05 535.5
#> 46 1486.0 4.720003e-04 292.0 1.180312e-04 9357.0
#> 47 3408.0 1.140617e-03 127.5 5.586232e-05 191.0
#> 48 755.0 2.432546e-04 586.0 2.202173e-04 122.0
#> 49 509.0 1.674370e-04 187.0 7.913835e-05 1289.0
#> 50 465.0 1.538049e-04 216.5 9.029187e-05 12307.0
#> 51 924.0 2.954040e-04 133.0 5.806795e-05 79.0
#> 52 357.0 1.200927e-04 196.0 8.256392e-05 8496.0
#> 53 644.0 2.090804e-04 389.0 1.524976e-04 8762.0
#> 54 1335.0 4.239250e-04 10780.0 5.274441e-03 12736.0
#> 55 173.0 6.087793e-05 105.0 4.669406e-05 59.0
#> 56 2524.5 8.190911e-04 105.5 4.690057e-05 55.0
#> 57 397.0 1.326313e-04 2108.0 7.350240e-04 9692.0
#> 58 467.5 1.545807e-04 20011.0 1.655050e-02 19606.0
#> 59 166.0 5.854283e-05 124.0 5.445190e-05 154.0
#> 60 388.0 1.298164e-04 3736.0 1.342727e-03 7436.0
#> 61 505.0 1.661994e-04 14386.0 8.533693e-03 2343.0
#> 62 693.0 2.241657e-04 141.0 6.125392e-05 79.0
#> 63 379.0 1.269980e-04 153.0 6.598696e-05 167.5
#> 64 238.0 8.219435e-05 174.0 7.415129e-05 181.0
#> 65 196.0 6.849067e-05 119.0 5.242741e-05 278.0
#> 66 677.0 2.192403e-04 12254.0 6.474164e-03 16011.0
#> 67 255.0 8.768139e-05 189.0 7.990143e-05 2247.5
#> 68 313.5 1.063610e-04 289.0 1.169469e-04 9351.5
#> 69 299.0 1.017565e-04 253.0 1.038282e-04 95.0
#> 70 131.0 4.671506e-05 198.0 8.332233e-05 135.5
#> 71 205.0 7.144701e-05 4646.5 1.720917e-03 2550.0
#> 72 250.0 8.607069e-05 96.0 4.295294e-05 68.0
#> 73 105.0 3.772725e-05 131.0 5.726739e-05 66.0
#> 74 234.5 8.106077e-05 66.0 3.010041e-05 75.5
#> 75 413.0 1.376275e-04 75.0 3.402553e-05 469.5
#> 76 141.0 5.012276e-05 7595.0 3.181729e-03 14464.0
#> 77 470.0 1.553563e-04 98.0 4.378833e-05 2319.5
#> 78 2021.0 6.469719e-04 10184.0 4.834414e-03 5805.5
#> 79 211.0 7.341150e-05 131.5 5.746769e-05 325.0
#> 80 793.0 2.549611e-04 172.0 7.337972e-05 6106.0
#> 81 1950.0 6.233034e-04 106.0 4.710695e-05 191.0
#> 82 791.0 2.543448e-04 169.0 7.222010e-05 66.0
#> 83 264.0 9.057441e-05 107.0 4.751929e-05 645.0
#> 84 259.0 8.896814e-05 101.0 4.503703e-05 7423.0
#> 85 673.0 2.128149e-04 327.0 1.217249e-04 182.0
#> 86 117.0 2.616968e-05 197.0 7.174298e-05 58.0
#> 87 208.0 5.796701e-05 374.0 1.392103e-04 221.5
#> 88 101.0 2.058224e-05 89.0 2.739027e-05 48.0
#> 89 299.0 8.916794e-05 507.0 1.876114e-04 209.5
#> 90 175.0 4.648858e-05 78.0 2.269013e-05 70.0
#> 91 529.0 1.657836e-04 149.0 5.243381e-05 962.0
#> 92 22.0 1.953125e-05 13.0 1.953125e-05 15.5
#> 93 22.0 1.953125e-05 13.0 1.953125e-05 16.0
#> 94 2508.5 8.214380e-04 8001.0 3.374298e-03 2342.0
#> 95 407.0 1.254594e-04 273.0 1.012971e-04 535.5
#> 96 892.0 2.837839e-04 288.0 1.070125e-04 306.0
#> 97 1130.5 3.609534e-04 83.0 2.482965e-05 100.0
#> 98 965.0 3.073909e-04 174.0 6.256314e-05 79.0
#> 99 335.5 1.015114e-04 140.0 4.874527e-05 390.0
#> 100 421.0 1.301176e-04 182.5 6.597041e-05 1777.5
#> 101 331.5 1.001629e-04 165.0 5.893564e-05 77.0
#> 102 235.0 6.729310e-05 135.0 4.668597e-05 3017.0
#> 103 315.0 9.458964e-05 210.0 7.687812e-05 2575.5
#> 104 409.0 1.261254e-04 7632.0 3.173026e-03 9860.0
#> 105 72.0 1.953125e-05 64.0 1.953125e-05 37.0
#> 106 37.0 1.953125e-05 25.0 1.953125e-05 26.5
#> 107 253.0 7.347746e-05 329.0 1.224741e-04 3973.0
#> 108 415.0 1.281223e-04 18287.0 1.207495e-02 11482.5
#> 109 117.0 2.616968e-05 129.0 4.420507e-05 168.0
#> 110 22.0 1.953125e-05 12.0 1.953125e-05 15.0
#> 111 326.0 9.830710e-05 1172.5 4.203235e-04 986.0
#> 112 221.0 6.246483e-05 191.0 6.936026e-05 89.0
#> 113 165.5 4.316929e-05 88.0 2.696408e-05 66.0
#> 114 275.0 8.100141e-05 230.5 8.490431e-05 137.0
#> 115 130.0 3.072718e-05 130.5 4.482630e-05 78.0
#> 116 401.0 1.234602e-04 5932.5 2.316812e-03 3536.0
#> 117 333.0 1.006687e-04 166.0 5.933973e-05 791.0
#> 118 241.5 6.952936e-05 173.0 6.216110e-05 1479.5
#> 119 126.0 2.932412e-05 67.0 1.953125e-05 48.0
#> 120 137.5 3.335844e-05 176.0 6.336645e-05 204.5
#> 121 368.0 1.124318e-04 10430.0 4.852997e-03 2340.0
#> 122 441.0 1.367566e-04 128.0 4.379054e-05 141.0
#> 123 152.5 3.861832e-05 147.0 5.161613e-05 90.0
#> 124 392.5 1.206250e-04 89.0 2.739027e-05 142.5
#> 125 438.0 1.357619e-04 173.0 6.216110e-05 507.0
#> 126 186.0 5.032408e-05 9767.0 4.421413e-03 15945.0
#> 127 1920.5 6.207170e-04 220.5 8.099961e-05 4833.0
#> 128 1993.0 6.450711e-04 12836.5 6.619260e-03 7426.0
#> 129 371.5 1.136043e-04 229.0 8.431984e-05 535.5
#> 130 1486.0 4.767496e-04 292.0 1.085310e-04 9357.0
#> 131 3408.0 1.144546e-03 127.5 4.358316e-05 191.0
#> 132 755.0 2.394355e-04 586.0 2.158224e-04 122.0
#> 133 509.0 1.592101e-04 187.0 6.776716e-05 1289.0
#> 134 465.0 1.447012e-04 216.5 7.943219e-05 12307.0
#> 135 924.0 2.941325e-04 133.0 4.586019e-05 79.0
#> 136 357.0 1.087426e-04 196.0 7.134643e-05 8496.0
#> 137 644.0 2.033776e-04 389.0 1.447421e-04 8762.0
#> 138 1335.0 4.273953e-04 10780.0 5.089917e-03 12736.0
#> 139 173.0 4.579029e-05 105.0 3.417289e-05 59.0
#> 140 2524.5 8.270079e-04 105.5 3.438367e-05 55.0
#> 141 397.0 1.221264e-04 2108.0 7.487858e-04 9692.0
#> 142 467.5 1.455275e-04 20011.0 1.434287e-02 19606.0
#> 143 166.0 4.334413e-05 124.0 4.212942e-05 154.0
#> 144 388.0 1.191225e-04 3736.0 1.363475e-03 7436.0
#> 145 505.0 1.578938e-04 14386.0 7.943317e-03 2343.0
#> 146 693.0 2.193157e-04 141.0 4.915625e-05 79.0
#> 147 379.0 1.161143e-04 153.0 5.406582e-05 167.5
#> 148 238.0 6.832565e-05 174.0 6.256314e-05 181.0
#> 149 196.0 5.380292e-05 119.0 4.004621e-05 278.0
#> 150 677.0 2.141155e-04 12254.0 6.161127e-03 16011.0
#> 151 255.0 7.416302e-05 189.0 6.856418e-05 2247.5
#> 152 313.5 9.408210e-05 289.0 1.073924e-04 9351.5
#> 153 299.0 8.916794e-05 253.0 9.362151e-05 95.0
#> 154 131.0 3.107799e-05 198.0 7.213931e-05 135.5
#> 155 205.0 5.692708e-05 4646.5 1.739516e-03 2550.0
#> 156 250.0 7.244854e-05 96.0 3.036641e-05 68.0
#> 157 105.0 2.197531e-05 131.0 4.503322e-05 66.0
#> 158 234.5 6.712094e-05 66.0 1.953125e-05 75.5
#> 159 413.0 1.274569e-04 75.0 2.140449e-05 469.5
#> 160 141.0 3.458626e-05 7595.0 3.153161e-03 14464.0
#> 161 470.0 1.463535e-04 98.0 3.121430e-05 2319.5
#> 162 2021.0 6.545047e-04 10184.0 4.690273e-03 5805.5
#> 163 211.0 5.900620e-05 131.5 4.524008e-05 325.0
#> 164 793.0 2.517479e-04 172.0 6.175882e-05 6106.0
#> 165 1950.0 6.306141e-04 106.0 3.459437e-05 191.0
#> 166 791.0 2.511002e-04 169.0 6.055043e-05 66.0
#> 167 264.0 7.724411e-05 107.0 3.501555e-05 645.0
#> 168 259.0 7.553317e-05 101.0 3.248401e-05 7423.0
#> 169 673.0 2.101268e-04 327.0 1.254895e-04 182.0
#> 170 117.0 4.367058e-05 197.0 8.003744e-05 58.0
#> 171 208.0 7.189714e-05 374.0 1.416271e-04 221.5
#> 172 101.0 3.851760e-05 89.0 4.005637e-05 48.0
#> 173 299.0 9.922093e-05 507.0 1.869559e-04 209.5
#> 174 175.0 6.181569e-05 78.0 3.572815e-05 70.0
#> 175 529.0 1.673173e-04 149.0 6.267963e-05 962.0
#> 176 22.0 1.953125e-05 13.0 1.953125e-05 15.5
#> 177 22.0 1.953125e-05 13.0 1.953125e-05 16.0
#> 178 2508.5 8.167212e-04 8001.0 3.834562e-03 2342.0
#> 179 407.0 1.312378e-04 273.0 1.067973e-04 535.5
#> 180 892.0 2.761533e-04 288.0 1.120099e-04 306.0
#> 181 1130.5 3.497158e-04 83.0 3.770492e-05 100.0
#> 182 965.0 2.984715e-04 174.0 7.177886e-05 79.0
#> 183 335.5 1.100688e-04 140.0 5.936601e-05 390.0
#> 184 421.0 1.353767e-04 182.5 7.484217e-05 1777.5
#> 185 331.5 1.088819e-04 165.0 6.851944e-05 77.0
#> 186 235.0 8.006235e-05 135.0 5.751515e-05 3017.0
#> 187 315.0 1.039812e-04 210.0 8.466618e-05 2575.5
#> 188 409.0 1.318292e-04 7632.0 3.590545e-03 9860.0
#> 189 72.0 2.891322e-05 64.0 3.009636e-05 37.0
#> 190 37.0 1.953125e-05 25.0 1.953125e-05 26.5
#> 191 253.0 8.547428e-05 329.0 1.261783e-04 3973.0
#> 192 415.0 1.336030e-04 18287.0 1.441355e-02 11482.5
#> 193 117.0 4.367058e-05 129.0 5.528397e-05 168.0
#> 194 22.0 1.953125e-05 12.0 1.953125e-05 15.0
#> 195 326.0 1.072492e-04 1172.5 4.161836e-04 986.0
#> 196 221.0 7.583644e-05 191.0 7.789207e-05 89.0
#> 197 165.5 5.888692e-05 88.0 3.966594e-05 66.0
#> 198 275.0 9.206174e-05 230.5 9.191699e-05 137.0
#> 199 130.0 4.780110e-05 130.5 5.584284e-05 78.0
#> 200 401.0 1.294637e-04 5932.5 2.561755e-03 3536.0
#> 201 333.0 1.093270e-04 166.0 6.888245e-05 791.0
#> 202 241.5 8.201924e-05 173.0 7.141753e-05 1479.5
#> 203 126.0 4.653494e-05 67.0 3.131643e-05 48.0
#> 204 137.5 5.016474e-05 176.0 7.250090e-05 204.5
#> 205 368.0 1.197002e-04 10430.0 5.640497e-03 2340.0
#> 206 441.0 1.412885e-04 128.0 5.491098e-05 141.0
#> 207 152.5 5.485556e-05 147.0 6.194518e-05 90.0
#> 208 392.5 1.269499e-04 89.0 4.005637e-05 142.5
#> 209 438.0 1.404018e-04 173.0 7.141753e-05 507.0
#> 210 186.0 6.519089e-05 9767.0 5.111783e-03 15945.0
#> 211 1920.5 6.082031e-04 220.5 8.838689e-05 4833.0
#> 212 1993.0 6.331484e-04 12836.5 7.807020e-03 7426.0
#> 213 371.5 1.207363e-04 229.0 9.138826e-05 535.5
#> 214 1486.0 4.630826e-04 292.0 1.133971e-04 9357.0
#> 215 3408.0 1.163860e-03 127.5 5.472436e-05 191.0
#> 216 755.0 2.346987e-04 586.0 2.138022e-04 122.0
#> 217 509.0 1.613966e-04 187.0 7.645843e-05 1289.0
#> 218 465.0 1.483829e-04 216.5 8.697125e-05 12307.0
#> 219 924.0 2.859160e-04 133.0 5.677269e-05 79.0
#> 220 357.0 1.164425e-04 196.0 7.968029e-05 8496.0
#> 221 644.0 2.014742e-04 389.0 1.467592e-04 8762.0
#> 222 1335.0 4.143601e-04 10780.0 5.931020e-03 12736.0
#> 223 173.0 6.120022e-05 105.0 4.623126e-05 59.0
#> 224 2524.5 8.225952e-04 105.5 4.642222e-05 55.0
#> 225 397.0 1.282808e-04 2108.0 7.615179e-04 9692.0
#> 226 467.5 1.491220e-04 20011.0 1.709641e-02 19606.0
#> 227 166.0 5.904141e-05 124.0 5.341565e-05 154.0
#> 228 388.0 1.256188e-04 3736.0 1.446722e-03 7436.0
#> 229 505.0 1.602129e-04 14386.0 9.426529e-03 2343.0
#> 230 693.0 2.161050e-04 141.0 5.973531e-05 79.0
#> 231 379.0 1.229560e-04 153.0 6.414542e-05 167.5
#> 232 238.0 8.096591e-05 174.0 7.177886e-05 181.0
#> 233 196.0 6.824594e-05 119.0 5.153858e-05 278.0
#> 234 677.0 2.113217e-04 12254.0 7.245290e-03 16011.0
#> 235 255.0 8.607428e-05 189.0 7.717560e-05 2247.5
#> 236 313.5 1.035353e-04 289.0 1.123568e-04 9351.5
#> 237 299.0 9.922093e-05 253.0 9.981731e-05 95.0
#> 238 131.0 4.811702e-05 198.0 8.039443e-05 135.5
#> 239 205.0 7.098576e-05 4646.5 1.881094e-03 2550.0
#> 240 250.0 8.457380e-05 96.0 4.277387e-05 68.0
#> 241 105.0 3.981391e-05 131.0 5.602897e-05 66.0
#> 242 234.5 7.991170e-05 66.0 3.091061e-05 75.5
#> 243 413.0 1.330118e-04 75.0 3.453394e-05 469.5
#> 244 141.0 5.126341e-05 7595.0 3.566495e-03 14464.0
#> 245 470.0 1.498612e-04 98.0 4.354557e-05 2319.5
#> 246 2021.0 6.428394e-04 10184.0 5.441058e-03 5805.5
#> 247 211.0 7.280763e-05 131.5 5.621502e-05 325.0
#> 248 793.0 2.461439e-04 172.0 7.105601e-05 6106.0
#> 249 1950.0 6.183277e-04 106.0 4.661308e-05 191.0
#> 250 791.0 2.455405e-04 169.0 6.997018e-05 66.0
#> 251 264.0 8.877137e-05 107.0 4.699446e-05 645.0
#> 252 259.0 8.727357e-05 101.0 4.469942e-05 7423.0
#> PvMSP8_Dilution PvRBP2b_MFI PvRBP2b_Dilution PvPTEX150_MFI
#> 1 5.436126e-05 1068.0 5.772191e-04 936.0
#> 2 1.953125e-05 465.5 2.523293e-04 122.0
#> 3 6.569336e-05 463.0 2.510122e-04 293.0
#> 4 1.953125e-05 591.0 3.183497e-04 109.0
#> 5 6.226189e-05 671.0 3.605866e-04 1665.5
#> 6 2.128036e-05 103.0 4.864448e-05 294.5
#> 7 2.752517e-04 1441.5 7.984089e-04 241.0
#> 8 1.953125e-05 14.0 1.953125e-05 28.0
#> 9 1.953125e-05 13.0 1.953125e-05 29.0
#> 10 6.996377e-04 1386.0 7.642147e-04 1992.0
#> 11 1.541357e-04 1575.0 8.827792e-04 8821.0
#> 12 8.966439e-05 590.0 3.178231e-04 315.0
#> 13 3.034480e-05 301.0 1.646050e-04 652.0
#> 14 2.402206e-05 1090.0 5.897088e-04 179.0
#> 15 1.133016e-04 6704.0 1.064529e-02 8814.0
#> 16 5.191597e-04 2390.0 1.475815e-03 2465.0
#> 17 2.341461e-05 244.0 1.331696e-04 579.0
#> 18 9.289961e-04 1034.0 5.580343e-04 4928.0
#> 19 7.772751e-04 2903.0 1.935858e-03 2779.0
#> 20 4.221942e-03 13703.0 2.000000e-02 5612.0
#> 21 1.953125e-05 156.0 8.215481e-05 204.0
#> 22 1.953125e-05 23.5 1.953125e-05 40.0
#> 23 1.280320e-03 1426.5 7.891177e-04 803.0
#> 24 5.304395e-03 12031.0 2.000000e-02 4830.5
#> 25 5.031588e-05 488.0 2.641746e-04 908.0
#> 26 1.953125e-05 14.0 1.953125e-05 29.0
#> 27 2.821752e-04 1041.0 5.619727e-04 299.5
#> 28 2.704492e-05 203.0 1.098937e-04 410.0
#> 29 2.005477e-05 205.0 1.110460e-04 200.0
#> 30 4.128794e-05 2032.0 1.197168e-03 1762.0
#> 31 2.371846e-05 903.0 4.853574e-04 1513.5
#> 32 1.115788e-03 4699.5 4.412617e-03 574.0
#> 33 2.262954e-04 1093.0 5.914166e-04 417.5
#> 34 4.278469e-04 899.0 4.831670e-04 1504.0
#> 35 1.953125e-05 400.0 2.177173e-04 183.0
#> 36 6.082943e-05 1090.0 5.897088e-04 504.0
#> 37 6.989804e-04 4375.0 3.830607e-03 1650.0
#> 38 4.245904e-05 1993.0 1.168643e-03 2147.0
#> 39 2.734596e-05 600.0 3.230899e-04 2178.0
#> 40 4.289769e-05 280.0 1.531229e-04 298.0
#> 41 1.461340e-04 1009.0 5.440161e-04 709.0
#> 42 9.031437e-03 10073.0 2.000000e-02 1585.5
#> 43 1.624070e-03 2340.0 1.434978e-03 5329.0
#> 44 2.831247e-03 11930.0 2.000000e-02 4826.0
#> 45 1.541357e-04 14032.0 2.000000e-02 7928.5
#> 46 3.912473e-03 8009.0 2.000000e-02 3736.0
#> 47 5.695312e-05 4401.0 3.874490e-03 11567.0
#> 48 3.687718e-05 830.0 4.456251e-04 425.5
#> 49 3.708318e-04 12930.0 2.000000e-02 13891.0
#> 50 5.906439e-03 5029.0 5.088849e-03 4157.0
#> 51 2.402206e-05 253.0 1.381944e-04 2317.5
#> 52 3.409934e-03 7914.0 2.000000e-02 5772.5
#> 53 3.561597e-03 9649.0 2.000000e-02 6437.0
#> 54 6.234291e-03 25375.5 2.000000e-02 5493.0
#> 55 1.953125e-05 480.0 2.599645e-04 655.0
#> 56 1.953125e-05 307.0 1.678679e-04 681.5
#> 57 4.117250e-03 8090.0 2.000000e-02 9513.0
#> 58 1.307769e-02 24476.5 2.000000e-02 5822.0
#> 59 4.625185e-05 224.0 1.219014e-04 718.0
#> 60 2.836428e-03 7361.0 1.452967e-02 4111.5
#> 61 6.999664e-04 5503.0 6.246341e-03 357.5
#> 62 2.402206e-05 339.0 1.851572e-04 2080.0
#> 63 5.017107e-05 1013.0 5.462541e-04 1553.0
#> 64 5.407287e-05 6160.0 8.328586e-03 1660.5
#> 65 8.175132e-05 787.0 4.224437e-04 2052.5
#> 66 9.095810e-03 18719.0 2.000000e-02 3551.0
#> 67 6.687202e-04 3322.0 2.376005e-03 590.0
#> 68 3.909155e-03 7171.0 1.325229e-02 3062.0
#> 69 2.884793e-05 712.0 3.823504e-04 396.0
#> 70 4.084825e-05 392.0 2.134698e-04 292.0
#> 71 7.687098e-04 2664.5 1.712216e-03 724.0
#> 72 2.066814e-05 1020.0 5.501751e-04 960.0
#> 73 2.005477e-05 1449.0 8.030684e-04 1724.5
#> 74 2.295835e-05 259.0 1.415299e-04 260.0
#> 75 1.356111e-04 802.0 4.305128e-04 345.0
#> 76 7.661842e-03 7592.0 1.629388e-02 804.5
#> 77 6.922503e-04 2009.0 1.180305e-03 1521.0
#> 78 2.045868e-03 14083.5 2.000000e-02 4307.5
#> 79 9.502199e-05 3021.0 2.053395e-03 4608.0
#> 80 2.183552e-03 2093.0 1.242472e-03 1303.0
#> 81 5.695312e-05 2411.0 1.493163e-03 7377.5
#> 82 2.005477e-05 990.0 5.334109e-04 261.0
#> 83 1.849476e-04 7092.0 1.276163e-02 8856.0
#> 84 2.829694e-03 1203.0 6.548540e-04 1841.5
#> 85 4.471844e-05 2208.0 1.504302e-03 936.0
#> 86 1.953125e-05 290.0 1.478949e-04 122.0
#> 87 5.574893e-05 376.0 1.953424e-04 293.0
#> 88 1.953125e-05 103.0 4.703989e-05 109.0
#> 89 5.239897e-05 650.0 3.528650e-04 1665.5
#> 90 1.953125e-05 293.5 1.498103e-04 294.5
#> 91 2.624386e-04 1632.5 1.021595e-03 241.0
#> 92 1.953125e-05 14.0 1.953125e-05 28.0
#> 93 1.953125e-05 13.0 1.953125e-05 29.0
#> 94 6.723443e-04 9995.5 2.000000e-02 1992.0
#> 95 1.430674e-04 7092.0 1.047171e-02 8821.0
#> 96 7.929950e-05 548.0 2.929884e-04 315.0
#> 97 2.187509e-05 443.0 2.329130e-04 652.0
#> 98 1.953125e-05 1092.0 6.317895e-04 179.0
#> 99 1.026535e-04 7093.0 1.047499e-02 8814.0
#> 100 4.994248e-04 1832.0 1.180540e-03 2465.0
#> 101 1.953125e-05 244.0 1.228302e-04 579.0
#> 102 8.898742e-04 5339.0 5.895922e-03 4928.0
#> 103 7.462262e-04 1029.0 5.899739e-04 2779.0
#> 104 3.907811e-03 7201.0 1.083354e-02 5612.0
#> 105 1.953125e-05 156.0 7.536840e-05 204.0
#> 106 1.953125e-05 23.5 1.953125e-05 40.0
#> 107 1.219504e-03 2812.0 2.097206e-03 803.0
#> 108 4.884853e-03 6291.0 8.115546e-03 4830.5
#> 109 4.080767e-05 315.0 1.616044e-04 908.0
#> 110 1.953125e-05 14.0 1.953125e-05 29.0
#> 111 2.692280e-04 902.0 5.078209e-04 299.5
#> 112 1.953125e-05 398.0 2.076171e-04 410.0
#> 113 1.953125e-05 174.0 8.503170e-05 200.0
#> 114 3.215431e-05 1903.7 1.239786e-03 1762.0
#> 115 1.953125e-05 492.1 2.608163e-04 1513.5
#> 116 1.065592e-03 4699.5 4.687478e-03 574.0
#> 117 2.143192e-04 653.0 3.546499e-04 417.5
#> 118 4.112034e-04 439.0 2.306539e-04 1504.0
#> 119 1.953125e-05 143.0 6.840218e-05 183.0
#> 120 5.100283e-05 210.0 1.044242e-04 504.0
#> 121 6.717176e-04 4375.0 4.147071e-03 1650.0
#> 122 3.326983e-05 431.0 2.261421e-04 2147.0
#> 123 1.953125e-05 299.0 1.528229e-04 2178.0
#> 124 3.368827e-05 323.5 1.662806e-04 298.0
#> 125 1.351489e-04 829.0 4.618583e-04 709.0
#> 126 8.243857e-03 10073.0 2.000000e-02 1585.5
#> 127 1.538972e-03 3718.0 3.183030e-03 5329.0
#> 128 2.646452e-03 14663.0 2.000000e-02 4826.0
#> 129 1.430674e-04 9105.0 1.929399e-02 7928.5
#> 130 3.627904e-03 7769.5 1.290600e-02 3736.0
#> 131 4.723245e-05 2401.0 1.683549e-03 11567.0
#> 132 2.797640e-05 398.0 2.076171e-04 425.5
#> 133 3.558277e-04 6039.5 7.473924e-03 13891.0
#> 134 5.427460e-03 3907.0 3.443708e-03 4157.0
#> 135 1.953125e-05 253.0 1.277187e-04 2317.5
#> 136 3.172539e-03 914.0 5.154632e-04 5772.5
#> 137 3.310090e-03 7649.0 1.243923e-02 6437.0
#> 138 5.722830e-03 9375.5 2.000000e-02 5493.0
#> 139 1.953125e-05 599.0 3.227327e-04 655.0
#> 140 1.953125e-05 491.0 2.601876e-04 681.5
#> 141 3.813157e-03 8115.0 1.433439e-02 9513.0
#> 142 1.190868e-02 9476.5 2.000000e-02 5822.0
#> 143 3.689788e-05 224.0 1.119918e-04 718.0
#> 144 2.651172e-03 2361.0 1.645641e-03 4111.5
#> 145 6.726577e-04 803.0 4.457041e-04 357.5
#> 146 1.953125e-05 995.0 5.677024e-04 2080.0
#> 147 4.066801e-05 480.5 2.541947e-04 1553.0
#> 148 4.443909e-05 904.5 5.094110e-04 1660.5
#> 149 7.150338e-05 321.0 1.649044e-04 2052.5
#> 150 8.301953e-03 18719.0 2.000000e-02 3551.0
#> 151 6.428435e-04 479.0 2.533398e-04 590.0
#> 152 3.624901e-03 771.0 4.259753e-04 3062.0
#> 153 2.049556e-05 803.0 4.457041e-04 396.0
#> 154 3.173611e-05 329.0 1.693105e-04 292.0
#> 155 7.380885e-04 2664.5 1.943565e-03 724.0
#> 156 1.953125e-05 702.0 3.840001e-04 960.0
#> 157 1.953125e-05 809.0 4.494220e-04 1724.5
#> 158 1.953125e-05 259.0 1.309818e-04 260.0
#> 159 1.247332e-04 388.0 2.020304e-04 345.0
#> 160 7.008869e-03 7592.0 1.222380e-02 804.5
#> 161 6.652996e-04 1904.5 1.240454e-03 1521.0
#> 162 1.928004e-03 9083.5 1.917057e-02 4307.5
#> 163 8.458592e-05 6791.0 9.526322e-03 4608.0
#> 164 2.054438e-03 5444.0 6.114352e-03 1303.0
#> 165 4.723245e-05 1411.0 8.548922e-04 7377.5
#> 166 1.953125e-05 899.0 5.059142e-04 261.0
#> 167 1.735326e-04 5201.0 5.617820e-03 8856.0
#> 168 2.645037e-03 2494.0 1.773257e-03 1841.5
#> 169 5.321878e-05 593.0 3.088593e-04 936.0
#> 170 1.957652e-05 128.0 6.043084e-05 122.0
#> 171 6.348963e-05 348.0 1.722321e-04 293.0
#> 172 1.953125e-05 180.0 8.601213e-05 109.0
#> 173 6.037974e-05 650.0 3.425772e-04 1665.5
#> 174 2.302049e-05 293.5 1.436247e-04 294.5
#> 175 2.563427e-04 532.5 2.738767e-04 241.0
#> 176 1.953125e-05 14.0 1.953125e-05 28.0
#> 177 1.953125e-05 13.0 1.953125e-05 29.0
#> 178 6.612912e-04 9995.5 2.000000e-02 1992.0
#> 179 1.440963e-04 12560.0 2.000000e-02 8821.0
#> 180 8.523428e-05 2060.0 1.428137e-03 315.0
#> 181 3.137212e-05 201.0 9.648033e-05 652.0
#> 182 2.555932e-05 709.0 3.782602e-04 179.0
#> 183 1.067420e-04 5769.5 7.451954e-03 8814.0
#> 184 4.873817e-04 1845.0 1.229413e-03 2465.0
#> 185 2.499799e-05 244.0 1.181812e-04 579.0
#> 186 8.847862e-04 5339.0 6.437161e-03 4928.0
#> 187 7.366725e-04 4592.5 4.907881e-03 2779.0
#> 188 4.192268e-03 9340.5 2.000000e-02 5612.0
#> 189 1.953125e-05 156.0 7.414759e-05 204.0
#> 190 1.953125e-05 23.5 1.953125e-05 40.0
#> 191 1.230916e-03 1412.0 8.668623e-04 803.0
#> 192 5.287991e-03 5816.0 7.567894e-03 4830.5
#> 193 4.955038e-05 315.0 1.548349e-04 908.0
#> 194 1.953125e-05 14.0 1.953125e-05 29.0
#> 195 2.628178e-04 1562.0 9.869116e-04 299.5
#> 196 2.834449e-05 293.5 1.436247e-04 410.0
#> 197 2.188070e-05 218.5 1.052681e-04 200.0
#> 198 4.135288e-05 729.5 3.908459e-04 1762.0
#> 199 2.527885e-05 440.0 2.219777e-04 1513.5
#> 200 1.068343e-03 4699.5 5.110429e-03 574.0
#> 201 2.107276e-04 621.0 3.253301e-04 417.5
#> 202 4.002525e-04 1037.5 5.917604e-04 1504.0
#> 203 1.953125e-05 270.0 1.314825e-04 183.0
#> 204 5.908153e-05 897.0 4.973376e-04 504.0
#> 205 6.606543e-04 4375.0 4.512229e-03 1650.0
#> 206 4.241750e-05 2119.5 1.485413e-03 2147.0
#> 207 2.862118e-05 399.0 1.995802e-04 2178.0
#> 208 4.281615e-05 323.5 1.592938e-04 298.0
#> 209 1.367559e-04 1584.0 1.005006e-03 709.0
#> 210 9.040032e-03 10073.0 2.000000e-02 1585.5
#> 211 1.572636e-03 3718.0 3.439588e-03 5329.0
#> 212 2.785456e-03 8663.0 1.768024e-02 4826.0
#> 213 1.440963e-04 8105.0 1.514557e-02 7928.5
#> 214 3.878930e-03 7769.5 1.377018e-02 3736.0
#> 215 5.556829e-05 901.0 4.999610e-04 11567.0
#> 216 3.733826e-05 529.0 2.718782e-04 425.5
#> 217 3.462083e-04 5039.5 5.790257e-03 13891.0
#> 218 5.896705e-03 8907.0 1.889422e-02 4157.0
#> 219 2.555932e-05 253.0 1.227699e-04 2317.5
#> 220 3.370323e-03 7914.0 1.434963e-02 5772.5
#> 221 3.523772e-03 4649.0 5.014172e-03 6437.0
#> 222 6.227858e-03 7375.5 1.228437e-02 5493.0
#> 223 1.986649e-05 599.0 3.123738e-04 655.0
#> 224 1.953125e-05 491.0 2.503564e-04 681.5
#> 225 4.086262e-03 6115.0 8.344521e-03 9513.0
#> 226 1.305624e-02 5476.5 6.750059e-03 5822.0
#> 227 4.586259e-05 224.0 1.080424e-04 718.0
#> 228 2.790686e-03 3361.0 2.927842e-03 4111.5
#> 229 6.616098e-04 2503.0 1.879237e-03 357.5
#> 230 2.555932e-05 1295.0 7.771891e-04 2080.0
#> 231 4.941903e-05 1280.5 7.663144e-04 1553.0
#> 232 5.295733e-05 1804.5 1.193399e-03 1660.5
#> 233 7.805039e-05 223.0 1.075375e-04 2052.5
#> 234 9.104432e-03 3142.0 2.636750e-03 3551.0
#> 235 6.313599e-04 1023.0 5.817978e-04 590.0
#> 236 3.875570e-03 873.0 4.816763e-04 3062.0
#> 237 3.000017e-05 203.0 9.748166e-05 396.0
#> 238 4.095304e-05 223.4 1.077394e-04 292.0
#> 239 7.283414e-04 664.5 3.512728e-04 724.0
#> 240 2.245155e-05 563.0 2.914093e-04 960.0
#> 241 2.188070e-05 406.0 2.033781e-04 1724.5
#> 242 2.457595e-05 259.0 1.258381e-04 260.0
#> 243 1.271180e-04 530.5 2.727344e-04 345.0
#> 244 7.666304e-03 7592.0 1.308416e-02 804.5
#> 245 6.541347e-04 2304.5 1.669986e-03 1521.0
#> 246 1.994700e-03 14083.5 2.000000e-02 4307.5
#> 247 9.010246e-05 2791.0 2.204322e-03 4608.0
#> 248 2.132952e-03 744.0 3.998065e-04 1303.0
#> 249 5.556829e-05 2411.0 1.780787e-03 7377.5
#> 250 2.188070e-05 412.0 2.066419e-04 261.0
#> 251 1.724537e-04 2381.0 1.749234e-03 8856.0
#> 252 2.783888e-03 1494.0 9.317742e-04 1841.5
#> PvPTEX150_Dilution PvCSS_MFI PvCSS_Dilution Location.2 QC_total
#> 1 8.126353e-04 223.0 2.841872e-04 B1 pass
#> 2 1.229486e-04 93.0 1.353705e-04 B2 pass
#> 3 2.781106e-04 868.0 8.856034e-04 B3 pass
#> 4 1.103123e-04 110.0 1.567278e-04 B4 pass
#> 5 1.423638e-03 266.0 3.287346e-04 B5 pass
#> 6 2.794140e-04 92.0 1.340863e-04 B6 pass
#> 7 2.324647e-04 282.0 3.449555e-04 B7 pass
#> 8 2.574652e-05 17.0 2.166211e-05 B8 pass
#> 9 2.687119e-05 17.0 2.166211e-05 B9 pass
#> 10 1.707722e-03 2927.0 2.805942e-03 B10 pass
#> 11 1.036353e-02 998.0 1.000714e-03 B11 pass
#> 12 2.971639e-04 366.0 4.277265e-04 B12 pass
#> 13 5.793013e-04 130.0 1.808769e-04 C1 pass
#> 14 1.765376e-04 92.0 1.340863e-04 C2 pass
#> 15 1.035121e-02 5627.0 6.009048e-03 C3 pass
#> 16 2.134204e-03 411.0 4.707921e-04 C4 pass
#> 17 5.190847e-04 229.0 2.904966e-04 C5 pass
#> 18 4.708546e-03 7730.0 9.302633e-03 C6 pass
#> 19 2.428062e-03 7300.0 8.560510e-03 C7 pass
#> 20 5.546200e-03 6383.0 7.099638e-03 C8 pass
#> 21 1.993268e-04 49.0 7.478312e-05 C9 pass
#> 22 3.910404e-05 27.0 3.959124e-05 C10 pass
#> 23 7.033817e-04 1670.0 1.600704e-03 C11 pass
#> 24 4.593868e-03 4217.0 4.214918e-03 C12 pass
#> 25 7.896158e-04 87.0 1.276134e-04 D1 pass
#> 26 2.687119e-05 17.0 2.166211e-05 D2 pass
#> 27 2.837541e-04 149.0 2.030322e-04 D3 pass
#> 28 3.781569e-04 174.5 2.318011e-04 D4 pass
#> 29 1.957042e-04 183.0 2.411822e-04 D5 pass
#> 30 1.506793e-03 1241.0 1.215908e-03 D6 pass
#> 31 1.293936e-03 325.0 3.877741e-04 D7 pass
#> 32 5.149503e-04 5389.0 5.685276e-03 D8 pass
#> 33 3.844806e-04 262.0 3.246518e-04 D9 pass
#> 34 1.285879e-03 260.0 3.226060e-04 D10 pass
#> 35 1.802086e-04 61.0 9.229740e-05 D11 pass
#> 36 4.568862e-04 459.0 5.160013e-04 D12 pass
#> 37 1.410342e-03 7801.5 9.429790e-03 E1 pass
#> 38 1.845438e-03 1502.0 1.448934e-03 E2 pass
#> 39 1.873214e-03 170.0 2.267950e-04 E3 pass
#> 40 2.824529e-04 138.0 1.902896e-04 E4 pass
#> 41 6.261848e-04 2040.0 1.941510e-03 E5 pass
#> 42 1.355185e-03 1124.0 1.112191e-03 E6 pass
#> 43 5.192471e-03 2369.0 2.253614e-03 E7 pass
#> 44 4.588603e-03 3468.5 3.373287e-03 E8 pass
#> 45 8.861825e-03 1414.5 1.370487e-03 E9 pass
#> 46 3.382304e-03 14370.5 2.000000e-02 E10 pass
#> 47 1.593738e-02 2923.5 2.802380e-03 E11 pass
#> 48 3.912167e-04 121.0 1.701260e-04 E12 pass
#> 49 2.000000e-02 9531.0 1.286590e-02 F1 pass
#> 50 3.832319e-03 760.0 7.895672e-04 F2 pass
#> 51 1.999194e-03 1080.5 1.073693e-03 F3 pass
#> 52 5.751431e-03 13202.0 2.000000e-02 F4 pass
#> 53 6.638026e-03 21113.0 2.000000e-02 F5 pass
#> 54 5.396205e-03 7696.0 9.242549e-03 F6 pass
#> 55 5.817712e-04 367.0 4.286918e-04 F7 pass
#> 56 6.035763e-04 472.5 5.286020e-04 F8 pass
#> 57 1.162533e-02 7341.0 8.629633e-03 F9 pass
#> 58 5.815412e-03 5127.5 5.339746e-03 F10 pass
#> 59 6.335804e-04 115.0 1.628552e-04 F11 pass
#> 60 3.782744e-03 2819.0 2.696607e-03 F12 pass
#> 61 3.336250e-04 1215.0 1.192832e-03 G1 pass
#> 62 1.785673e-03 1470.0 1.420201e-03 G2 pass
#> 63 1.327497e-03 1738.0 1.662620e-03 G3 pass
#> 64 1.419347e-03 1087.0 1.079444e-03 G4 pass
#> 65 1.761248e-03 2221.0 2.112066e-03 G5 pass
#> 66 3.190584e-03 10222.5 1.445612e-02 G6 pass
#> 67 5.281751e-04 258.0 3.205574e-04 G7 pass
#> 68 2.700773e-03 676.5 7.148157e-04 G8 pass
#> 69 3.663289e-04 129.5 1.802842e-04 G9 pass
#> 70 2.772412e-04 254.5 3.169650e-04 G10 pass
#> 71 6.385101e-04 2707.0 2.584492e-03 G11 pass
#> 72 8.323787e-04 672.0 7.107699e-04 G12 pass
#> 73 1.474402e-03 108.0 1.542585e-04 H1 pass
#> 74 2.492539e-04 89.0 1.302131e-04 H2 pass
#> 75 3.229441e-04 744.5 7.757318e-04 H3 pass
#> 76 7.046131e-04 467.0 5.234739e-04 H4 pass
#> 77 1.300300e-03 1258.0 1.231007e-03 H5 pass
#> 78 3.997956e-03 3073.0 2.955711e-03 H6 pass
#> 79 4.336431e-03 1073.5 1.067500e-03 H7 pass
#> 80 1.116657e-03 6595.5 7.424131e-03 H8 pass
#> 81 8.000601e-03 1699.0 1.627073e-03 H9 pass
#> 82 2.501338e-04 65.0 9.794103e-05 H10 pass
#> 83 1.042524e-02 5330.0 5.606395e-03 H11 pass
#> 84 1.575799e-03 335.0 3.975899e-04 H12 pass
#> 85 8.064505e-04 223.0 2.839737e-04 B1 pass
#> 86 1.217169e-04 93.0 1.331903e-04 B2 pass
#> 87 2.845489e-04 868.0 8.933141e-04 B3 pass
#> 88 1.079354e-04 110.0 1.547665e-04 B4 pass
#> 89 1.375007e-03 266.0 3.292099e-04 B5 pass
#> 90 2.858801e-04 92.0 1.318941e-04 B6 pass
#> 91 2.376131e-04 282.0 3.456843e-04 B7 pass
#> 92 1.953125e-05 17.0 1.963813e-05 B8 pass
#> 93 1.953125e-05 17.0 1.963813e-05 B9 pass
#> 94 1.634444e-03 2927.0 2.794369e-03 B10 pass
#> 95 9.259962e-03 998.0 1.009285e-03 B11 pass
#> 96 3.039626e-04 366.0 4.297458e-04 B12 pass
#> 97 5.830856e-04 130.0 1.791998e-04 C1 pass
#> 98 1.791168e-04 92.0 1.318941e-04 C2 pass
#> 99 9.249152e-03 5627.0 5.828933e-03 C3 pass
#> 100 2.020234e-03 411.0 4.734686e-04 C4 pass
#> 101 5.245398e-04 229.0 2.903796e-04 C5 pass
#> 102 4.303548e-03 7730.0 8.812164e-03 C6 pass
#> 103 2.284114e-03 7300.0 8.150400e-03 C7 pass
#> 104 5.039023e-03 6383.0 6.830436e-03 C8 pass
#> 105 2.031021e-04 49.0 7.225796e-05 C9 pass
#> 106 2.789031e-05 27.0 3.723470e-05 C10 pass
#> 107 7.024741e-04 1670.0 1.609527e-03 C11 pass
#> 108 4.202705e-03 4217.0 4.147844e-03 C12 pass
#> 109 7.846211e-04 87.0 1.253631e-04 D1 pass
#> 110 1.953125e-05 17.0 1.963813e-05 D2 pass
#> 111 2.903093e-04 149.0 2.016418e-04 D3 pass
#> 112 3.855193e-04 174.5 2.308100e-04 D4 pass
#> 113 1.993040e-04 183.0 2.403264e-04 D5 pass
#> 114 1.451193e-03 1241.0 1.225393e-03 D6 pass
#> 115 1.255709e-03 325.0 3.891729e-04 D7 pass
#> 116 5.205035e-04 5389.0 5.528764e-03 D8 pass
#> 117 3.918289e-04 262.0 3.250634e-04 D9 pass
#> 118 1.248278e-03 260.0 3.229858e-04 D10 pass
#> 119 1.829957e-04 61.0 8.981493e-05 D11 pass
#> 120 4.635666e-04 459.0 5.193491e-04 D12 pass
#> 121 1.362804e-03 7801.5 8.924987e-03 E1 pass
#> 122 1.759445e-03 1502.0 1.458370e-03 E2 pass
#> 123 1.784603e-03 170.0 2.257326e-04 E3 pass
#> 124 2.889819e-04 138.0 1.887317e-04 E4 pass
#> 125 6.283792e-04 2040.0 1.947318e-03 E5 pass
#> 126 1.312120e-03 1124.0 1.121350e-03 E6 pass
#> 127 4.728653e-03 2369.0 2.254719e-03 E7 pass
#> 128 4.198074e-03 3468.5 3.343204e-03 E8 pass
#> 129 7.943202e-03 1414.5 1.380060e-03 E9 pass
#> 130 3.133692e-03 14370.5 2.000000e-02 E10 pass
#> 131 1.417747e-02 2923.5 2.790906e-03 E11 pass
#> 132 3.985416e-04 121.0 1.683183e-04 E12 pass
#> 133 1.963039e-02 9531.0 1.191604e-02 F1 pass
#> 134 3.531676e-03 760.0 7.963681e-04 F2 pass
#> 135 1.898508e-03 1080.5 1.082677e-03 F3 pass
#> 136 5.218987e-03 13202.0 2.000000e-02 F4 pass
#> 137 5.995805e-03 21113.0 2.000000e-02 F5 pass
#> 138 4.907445e-03 7696.0 8.758797e-03 F6 pass
#> 139 5.854778e-04 367.0 4.307259e-04 F7 pass
#> 140 6.065671e-04 472.5 5.321329e-04 F8 pass
#> 141 1.036852e-02 7341.0 8.212280e-03 F9 pass
#> 142 5.275077e-03 5127.5 5.206918e-03 F10 pass
#> 143 6.355028e-04 115.0 1.609627e-04 F11 pass
#> 144 3.487895e-03 2819.0 2.687973e-03 F12 pass
#> 145 3.408586e-04 1215.0 1.202263e-03 G1 pass
#> 146 1.705253e-03 1470.0 1.429701e-03 G2 pass
#> 147 1.286636e-03 1738.0 1.671064e-03 G3 pass
#> 148 1.371070e-03 1087.0 1.088456e-03 G4 pass
#> 149 1.683081e-03 2221.0 2.115530e-03 G5 pass
#> 150 2.963704e-03 10222.5 1.326578e-02 G6 pass
#> 151 5.334068e-04 258.0 3.209052e-04 G7 pass
#> 152 2.527908e-03 676.5 7.207929e-04 G8 pass
#> 153 3.736969e-04 129.5 1.785998e-04 G9 pass
#> 154 2.836607e-04 254.5 3.172569e-04 G10 pass
#> 155 6.402481e-04 2707.0 2.578654e-03 G11 pass
#> 156 8.251423e-04 672.0 7.166998e-04 G12 pass
#> 157 1.421542e-03 108.0 1.522701e-04 H1 pass
#> 158 2.549517e-04 89.0 1.279856e-04 H2 pass
#> 159 3.300830e-04 744.5 7.823877e-04 H3 pass
#> 160 7.036515e-04 467.0 5.269305e-04 H4 pass
#> 161 1.261578e-03 1258.0 1.240521e-03 H5 pass
#> 162 3.677856e-03 3073.0 2.939778e-03 H6 pass
#> 163 3.976151e-03 1073.5 1.076453e-03 H7 pass
#> 164 1.091593e-03 6595.5 7.125693e-03 H8 pass
#> 165 7.188888e-03 1699.0 1.635744e-03 H9 pass
#> 166 2.558578e-04 65.0 9.548421e-05 H10 pass
#> 167 9.314129e-03 5330.0 5.455429e-03 H11 pass
#> 168 1.514254e-03 335.0 3.991418e-04 H12 pass
#> 169 8.215151e-04 223.0 2.776873e-04 B1 pass
#> 170 1.195065e-04 93.0 1.316270e-04 B2 pass
#> 171 2.804068e-04 868.0 9.002537e-04 B3 pass
#> 172 1.062510e-04 110.0 1.521145e-04 B4 pass
#> 173 1.432302e-03 266.0 3.225301e-04 B5 pass
#> 174 2.817463e-04 92.0 1.304018e-04 B6 pass
#> 175 2.333873e-04 282.0 3.389487e-04 B7 pass
#> 176 1.953125e-05 17.0 2.715888e-05 B8 pass
#> 177 1.953125e-05 17.0 2.715888e-05 B9 pass
#> 178 1.715710e-03 2927.0 2.908299e-03 B10 pass
#> 179 1.064394e-02 998.0 1.021359e-03 B11 pass
#> 180 2.999716e-04 366.0 4.233442e-04 B12 pass
#> 181 5.867699e-04 130.0 1.755095e-04 C1 pass
#> 182 1.754436e-04 92.0 1.304018e-04 C2 pass
#> 183 1.063074e-02 5627.0 6.091068e-03 C3 pass
#> 184 2.141402e-03 411.0 4.675927e-04 C4 pass
#> 185 5.259093e-04 229.0 2.840147e-04 C5 pass
#> 186 4.735474e-03 7730.0 9.144823e-03 C6 pass
#> 187 2.435132e-03 7300.0 8.474140e-03 C7 pass
#> 188 5.591675e-03 6383.0 7.124960e-03 C8 pass
#> 189 1.991035e-04 49.0 7.482238e-05 C9 pass
#> 190 3.134009e-05 27.0 4.299915e-05 C10 pass
#> 191 7.117806e-04 1670.0 1.653317e-03 C11 pass
#> 192 4.618756e-03 4217.0 4.336311e-03 C12 pass
#> 193 7.984160e-04 87.0 1.242385e-04 D1 pass
#> 194 1.953125e-05 17.0 2.715888e-05 D2 pass
#> 195 2.862054e-04 149.0 1.971602e-04 D3 pass
#> 196 3.827983e-04 174.5 2.255062e-04 D4 pass
#> 197 1.953472e-04 183.0 2.348005e-04 D5 pass
#> 198 1.515264e-03 1241.0 1.248101e-03 D6 pass
#> 199 1.302868e-03 325.0 3.824892e-04 D7 pass
#> 200 5.217253e-04 5389.0 5.779485e-03 D8 pass
#> 201 3.892448e-04 262.0 3.184046e-04 D9 pass
#> 202 1.294825e-03 260.0 3.163386e-04 D10 pass
#> 203 1.792597e-04 61.0 9.101184e-05 D11 pass
#> 204 4.628790e-04 459.0 5.142431e-04 D12 pass
#> 205 1.419036e-03 7801.5 9.258789e-03 E1 pass
#> 206 1.853116e-03 1502.0 1.493623e-03 E2 pass
#> 207 1.880835e-03 170.0 2.205564e-04 E3 pass
#> 208 2.848687e-04 138.0 1.846874e-04 E4 pass
#> 209 6.340615e-04 2040.0 2.010850e-03 E5 pass
#> 210 1.363998e-03 1124.0 1.138790e-03 E6 pass
#> 211 5.229325e-03 2369.0 2.336583e-03 E7 pass
#> 212 4.613400e-03 3468.5 3.488677e-03 E8 pass
#> 213 9.045450e-03 1414.5 1.411000e-03 E9 pass
#> 214 3.392662e-03 14370.5 2.000000e-02 E10 pass
#> 215 1.675947e-02 2923.5 2.904632e-03 E11 pass
#> 216 3.961087e-04 121.0 1.650664e-04 E12 pass
#> 217 2.000000e-02 9531.0 1.224113e-02 F1 pass
#> 218 3.846615e-03 760.0 7.994231e-04 F2 pass
#> 219 2.006581e-03 1080.5 1.098225e-03 F3 pass
#> 220 5.802441e-03 13202.0 2.000000e-02 F4 pass
#> 221 6.717498e-03 21113.0 2.000000e-02 F5 pass
#> 222 5.437882e-03 7696.0 9.090877e-03 F6 pass
#> 223 5.892632e-04 367.0 4.243337e-04 F7 pass
#> 224 6.112656e-04 472.5 5.272777e-04 F8 pass
#> 225 1.200339e-02 7341.0 8.537015e-03 F9 pass
#> 226 5.868229e-03 5127.5 5.444541e-03 F10 pass
#> 227 6.415149e-04 115.0 1.580288e-04 F11 pass
#> 228 3.796524e-03 2819.0 2.795637e-03 F12 pass
#> 229 3.373220e-04 1215.0 1.223779e-03 G1 pass
#> 230 1.793482e-03 1470.0 1.463366e-03 G2 pass
#> 231 1.336365e-03 1738.0 1.718394e-03 G3 pass
#> 232 1.428021e-03 1087.0 1.104285e-03 G4 pass
#> 233 1.769111e-03 2221.0 2.189075e-03 G5 pass
#> 234 3.199757e-03 10222.5 1.356325e-02 G6 pass
#> 235 5.351064e-04 258.0 3.142704e-04 G7 pass
#> 236 2.708151e-03 676.5 7.211338e-04 G8 pass
#> 237 3.707335e-04 129.5 1.749327e-04 G9 pass
#> 238 2.795133e-04 254.5 3.106456e-04 G10 pass
#> 239 6.464820e-04 2707.0 2.679843e-03 G11 pass
#> 240 8.413187e-04 672.0 7.169026e-04 G12 pass
#> 241 1.482949e-03 108.0 1.497354e-04 H1 pass
#> 242 2.507076e-04 89.0 1.267114e-04 H2 pass
#> 243 3.263921e-04 744.5 7.849182e-04 H3 pass
#> 244 7.130191e-04 467.0 5.219714e-04 H4 pass
#> 245 1.309221e-03 1258.0 1.264015e-03 H5 pass
#> 246 4.014126e-03 3073.0 3.062203e-03 H6 pass
#> 247 4.357169e-03 1073.5 1.091700e-03 H7 pass
#> 248 1.125829e-03 6595.5 7.428082e-03 H8 pass
#> 249 8.138325e-03 1699.0 1.681038e-03 H9 pass
#> 250 2.516144e-04 65.0 9.627226e-05 H10 pass
#> 251 1.071008e-02 5330.0 5.703244e-03 H11 pass
#> 252 1.584103e-03 335.0 3.925077e-04 H12 pass
#>
#> [[2]]
#> NULL
#>
#> [[3]]
#>
#> [[4]]
#> # A tibble: 2 × 4
#> Location SampleID Plate QC
#> <chr> <chr> <chr> <chr>
#> 1 A1 Blank1 plate2 fail
#> 2 A2 Blank2 plate2 fail
#>
#> [[5]]
#>
#> [[6]]
#> [[6]]$`1`
#>
#> [[6]]$`2`
#>
#> [[6]]$`3`
#>
#>
# }